MeSH Browser

MeSH Heading: Two-Hybrid System Techniques
Tree Number(s): E05.393.220.870; E05.601.690.650; E05.601.870
Unique ID: D020798
RDF Unique Identifier: http://id.nlm.nih.gov/mesh/D020798
Scope Note: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Entry Term(s): One-Hybrid System Technics; One-Hybrid System Techniques; Reverse One-Hybrid System Techniques; Reverse Three-Hybrid System Techniques; Reverse Two-Hybrid System Techniques; Three-Hybrid System Technics; Three-Hybrid System Techniques; Tri-Hybrid System Techniques; Two-Hybrid Assay; Two-Hybrid Method; Two-Hybrid System Technics; Yeast One-Hybrid System Techniques; Yeast Three-Hybrid Assay; Yeast Three-Hybrid System; Yeast Three-Hybrid System Techniques; Yeast Two-Hybrid Assay; Yeast Two-Hybrid System; Yeast Two-Hybrid System Techniques; n-Hybrid System Techniques
See Also: Blotting, Far-Western
Public MeSH Note: 2000
History Note: 2000
Date Introduced: 2000/01/01
Last Updated: 2015/06/23

Allowable Qualifiers: adverse effects (AE)

classification (CL)

economics (EC)

ethics (ES)

history (HI)

instrumentation (IS)

mortality (MO)

nursing (NU)

psychology (PX)

standards (ST)

statistics & numerical data (SN)

trends (TD)

veterinary (VE)

Concept UI: M0328080
Scope Note: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Terms: Two-Hybrid System Techniques Preferred Term
Term UI T358080
Date10/12/1999
LexicalTag NON
ThesaurusID NLM (2000); Two-Hybrid Assay
Term UI T361955
Date10/12/1999
LexicalTag NON
ThesaurusID NLM (2000); Two-Hybrid Method
Term UI T361956
Date10/12/1999
LexicalTag NON
ThesaurusID NLM (2000); Two-Hybrid System Technics
Term UI T361957
Date10/12/1999
LexicalTag NON
ThesaurusID NLM (2000)

Two-Hybrid System Techniques MeSH Descriptor Data 2026