MeSH Browser

MeSH Heading: Microscopy, Fluorescence, Multiphoton
Tree Number(s): E01.370.350.515.458.500; E01.370.350.515.717.250; E05.595.458.500; E05.595.717.250
Unique ID: D036641
RDF Unique Identifier: http://id.nlm.nih.gov/mesh/D036641
Scope Note: Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
Entry Term(s): Fluorescence Microscopy, Multiphoton; Multiphoton Excitation Microscopy; Multiphoton Fluorescence Microscopy
Previous Indexing: Microscopy, Confocal (1997-2002); Microscopy, Fluorescence (1996-2002)
Public MeSH Note: 2003
History Note: 2003
Date Established: 2003/01/01
Date of Entry: 2002/07/03
Revision Date: 2017/06/30

Allowable Qualifiers: adverse effects (AE)

classification (CL)

economics (EC)

ethics (ES)

history (HI)

instrumentation (IS)

methods (MT)

mortality (MO)

nursing (NU)

psychology (PX)

standards (ST)

statistics & numerical data (SN)

trends (TD)

veterinary (VE)

Concept UI: M0410252
Scope Note: Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
Terms: Microscopy, Fluorescence, Multiphoton Preferred Term
Term UI T476986
Date01/10/2002
LexicalTag NON
ThesaurusID NLM (2003); Multiphoton Excitation Microscopy
Term UI T000912829
Date12/13/2016
LexicalTag NON
ThesaurusID NLM (2018); Multiphoton Fluorescence Microscopy
Term UI T477440
Date01/14/2002
LexicalTag NON
ThesaurusID NLM (2003); Fluorescence Microscopy, Multiphoton
Term UI T477443
Date01/14/2002
LexicalTag NON
ThesaurusID NLM (2003)

Microscopy, Fluorescence, Multiphoton MeSH Descriptor Data 2025