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Heteroduplex Analysis MeSH Descriptor Data 2022


MeSH Heading
Heteroduplex Analysis
Tree Number(s)
E05.393.661.250
Unique ID
D020180
RDF Unique Identifier
http://id.nlm.nih.gov/mesh/D020180
Scope Note
A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.
Entry Version
HETERODUPLEX ANAL
Previous Indexing
Nucleic Acid Heteroduplexes (1980-1998)
Nucleic Acid Hybridization (1973-1979)
See Also
Base Pair Mismatch
Nucleic Acid Heteroduplexes
Public MeSH Note
99
History Note
99
Date Established
1999/01/01
Date of Entry
1998/06/10
Heteroduplex Analysis Preferred
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