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Ligase Chain Reaction MeSH Descriptor Data 2023


MeSH Heading
Ligase Chain Reaction
Tree Number(s)
E05.393.620.311
Unique ID
D022521
RDF Unique Identifier
http://id.nlm.nih.gov/mesh/D022521
Scope Note
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Previous Indexing
Gene Amplification (1980-2000)
See Also
Polymerase Chain Reaction
Public MeSH Note
2001
History Note
2001
Date Established
2001/01/01
Date of Entry
2000/06/22
Ligase Chain Reaction Preferred
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